Mudit Tyagi, PhD
Associate Professor, Department of Medicine
Contact Information
Associate Professor, Department of Medicine
Expertise & Research Interests
Our Lab research revolves around HIV and drugs of abuse focusing mainly transcriptional and epigenetic aspects.
Dr. Tyagi received his PhD in the field of Molecular Biology of HIV from International center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
His main research areas of interest are:
1. Define the underlying molecular mechanisms that regulate HIV transcription and latency, and how can those mechanisms be used for therapeutic benefits.
2. Characterize the molecular mechanisms that different drugs of abuse modulate to enhance HIV replication and neurocognitive complications.
The main techniques we utilize are RNA-Seq, ChIP-Seq, ATAC-Seq Western blotting, cloning, flowcytometry, mutagenesis, human cell culture (primary and cell lines), protein expression and purification, enzymatic assays, light and fluorescent microscopy, confocal microscopy, transcriptomic and proteomic analyses, transcription and signal transduction assays, besides other usual molecular biology lab assays.
Publications
- Cocaine-Induced DNA-Dependent Protein Kinase Relieves RNAP II Pausing by Promoting TRIM28 Phosphorylation and RNAP II Hyperphosphorylation to Enhance HIV Transcription
- New Insights into HIV Life Cycle, Th1/Th2 Shift during HIV Infection and Preferential Virus Infection of Th2 Cells: Implications of Early HIV Treatment Initiation and Care
- An overview of the mechanisms of HIV-1 infection, latency, pathogenesis, and eradication strategies from the CNS
- Comparison of the Biological Basis for Non-HIV Transmission to HIV-Exposed Seronegative Individuals, Disease Non-Progression in HIV Long-Term Non-Progressors and Elite Controllers
- Cocaine sensitizes the CD4+ T cells for HIV infection by co-stimulating NFAT and AP-1